BGI 5090 PDF

/17/$ © IEEE Our proposed pipeline is implemented on BGI Online to provide a user-friendly graphical interface Index Terms—pipeline, single cell sequencing, copy number variation detection, BGI Online. ISBN: pp: Yuwen Zhou, BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China. Aodan Xu. (4)BGI Genomics, BGI-Shenzhen, Shenzhen, , China. association study on pulmonary TB patients and healthy controls.

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Despite the fact that the rice and mouse 50090 have similar amounts of raw input data, i. For our first benchmark test dataset, we used rice transcriptome data from Oryza sativa panicle at booting stage.

To cater for fast and convenient needs in calling copy-number variations in analyzing single-cell sequencing data, a systematical protocol and a working pipeline is reported.

Reference SNP (refSNP) Cluster Report: rs

As a result, this increases its ability to identify alternative splicing events Fig. Every module in the pipeline is designed to achieve unitary task, and is unattached, thus facilitating user-customized applications.

Series-A includes all assembled transcripts, while series-B is a strict subset that includes only the largest assembled transcript for any given gene.

The expectation is for these contigs to be linearly integrated into a single scaffold; however, for transcriptomes, conflicts bhi legitimately arise because multiple alternative splice forms share the same starting contig.

Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese.

The only way to avoid a misleading isoform count is to record only what had previously been annotated. We evaluated its performance on transcriptome datasets from rice and mouse. In the case of the rice transcriptome, about In contrast, transcriptome assemblers must recover an unknown number of RNA sequences, typically on the order of tens of thousands.

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Articles by Hongmin Cai. The results here demonstrated that SOAPdenovo-Trans provides higher contiguity, lower redundancy and faster execution. Articles by Enhong Zhuo. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. The second benchmark test dataset was mouse transcriptome data from Mus musculus dendritic cells.

Paired-end sequences were generated on an Illumina GA platform Zhang et al. Owing to the rapid increase in throughputs and decrease in costs of next- generation sequencing, RNA-Seq in particular has become the method of choice.

These programs were intended to recover sequences for genomes of a known estimated size with a defined number of chromosomes.

Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese.

The reference genomes and curated annotations were downloaded from the following two Web sites. DBG are constructed from reads; sequencing errors are removed; and contigs are then constructed. Bgj gene expression levels make it impossible to define a contig as repetitive using a single depth constant. The number of reads is then used to assign weights to these bhi, and insert sizes from the paired-ends are used to estimate the distances between linkages. We bbgi the japonica genome as a reference because these annotations are more extensively manually curated than their indica counterparts.

Given a set of assembled transcripts aligning to the same genome locus, L submaximal is the length of any transcript other than the largest, while L maximal is the length of the largest transcript.

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Finally, we used the same method as SOAPdenovo2 to generate contigs. Assemblers such as Cufflinks Trapnell et al. Here, the L dataset contained Thus, its error-removal model is not applicable to RNA-Seq data.

We do, however, note that there are local regions of higher variability that will prevent some indica transcripts from aligning to the japonica genome. L overlap is the length of the overlap between the two. Here, we only counted the isoforms that had been recorded in the genome annotations. However, for the most highly expressed genes in a transcriptome, sequencing errors often generate k -mers that exceed any reasonable global error removal threshold.

We used the terms series-A and series-B to denote the sets of transcripts that included or excluded putative alternative splice forms, respectively. We chose to assess both plant and bgl transcriptomes because most other studies only assessed animals or even simpler organisms like yeastand we wanted to be sure that our assembler could handle the difficulties 590 by plant data. All assemblies were processed with 10 threads, on a computer with two Quad-core Intel 2.

Current multiple k -mers assembly strategies generally fall into one of the two categories: However, in practice, the overlap between the assembled and annotated transcript is almost always perfect Fig.

Articles by Jingying Huang.